Nuzulia Irawati
Departement of Parasitology, Faculty of Medicine, Universitas Andalas, Padang 25127, Indonesia

Yanwirasti
Departement of Anatomy, Faculty of Medicine, Universitas Andalas, Padang 25127, Indonesia

Noza Hilbertina
Departement of Anatomical Pathology, Faculty of Medicine, Universitas Andalas, Padang 25127, Indonesia

DOI: https://doi.org/10.19184/ams.v10i3.47044

Abstract

Blastocystis sp. is one of the intestinal protozoa that is frequently found in stool specimens of colorectal cancer patients. The clinical aspects of this protozoan are still very controversial. The debate on Blastocystis sp. as a commensal, protecting, or aggravating colorectal cancer still continues. Increased expression of malondialdhyde, a marker of oxidative stress, is associated with the pathogenesis and progressivity of various diseases, including cancer. This study aims to determine the exposure of Blastocystis sp. on malondialdehyde expression in colorectal cancer cell line HT29. This research is an experimental study in vitro. HT29 cells were treated with various concentrations of Blastocystis sp. antigen (0.005 μg/ml, 0.01 μg/ml, 0.05 μg/ml, 0.1 μg/ml and 0.5 μg/ml) for 72 hours. Malondialdehyde expression was examined using spectrophotometric method. The results of statistical analysis using the Kruskall Wallis test showed that there were differences in malondialdehyde expression in HT29 cells after being exposed to Blastocystis sp. in various concentration (p=0.007). Post Hoc test showed that significant differences in malondialdehyde expression were found in the exposure groups of 0.1 and 0.5 μg/ml with the control group, 0.005 and 0.01 μg/ml with 0.1 μg/ml and groups of 0.005 and 0.01 μg/ml with 0.5 μg/ml. So it can be concluded that exposure to Blastocystis sp. with a certain intensity can suppress oxidative stress so that it can protect and inhibit the progressivity of cancer.

Keyword: Blastocystis sp., HT29, malondialdehyde.

REFERENCE

1. Ali, S. H., Ismail, M. A. M., El-Badry, A. A., Abu-Sarea, E. Y., Dewidar, A. M., & Hamdy, D. A. (2022). An Association Between Blastocystis Subtypes and Colorectal Cancer Patients: A Significant Different Profile from Non-cancer Individuals. Acta Parasitologica, 67(2), 752. https://doi.org/10.1007/S11686-021-00508-Y

2. Alief Gusnirwan Sulaiman, P. R. A. S. (n.d.). View of Malondialdehyde (MDA) as a Marker of Oxidative Stress in Various Diseases. Medical Profession Journal of Lampung Vol. 14 No. 2 (2024). https://doi.org/10.53089/medula.v14i2.990

3. Arfin, S., Jha, N. K., Jha, S. K., Kesari, K. K., Ruokolainen, J., Roychoudhury, S., Rathi, B., & Kumar, D. (2021). Oxidative Stress in Cancer Cell Metabolism. Antioxidants 2021, Vol. 10, Page 642, 10(5), 642. https://doi.org/10.3390/ANTIOX10050642

4. Aykur, M., Malatyalı, E., Demirel, F., Cömert-Koçak, B., Gentekaki, E., Tsaousis, A. D., & Dogruman-Al, F. (2024). Blastocystis: A Mysterious Member of the GutMicrobiome. Microorganisms, 12(3). https://doi.org/10.3390/MICROORGANISMS12030461

5. Bardelčíková, A., Šoltys, J., & Mojžiš, J. (2023). Oxidative Stress, Inflammation and Colorectal Cancer: An Overview. Antioxidants, 12(4). https://doi.org/10.3390/ANTIOX12040901

6. Chandramathi, S., Suresh, K., Sivanandam, S., & Kuppusamy, U. R. (2014). Stress Exacerbates Infectivity and Pathogenicity of Blastocystis hominis: In Vitro and In Vivo Evidences. PLoS ONE, 9(5). https://doi.org/10.1371/JOURNAL.PONE.0094567

7. Cordiano, R., Di Gioacchino, M., Mangifesta, R., Panzera, C., Gangemi, S., & Minciullo, P. L. (2023). Malondialdehyde as a Potential Oxidative Stress Marker for Allergy-Oriented Diseases: An Update. Molecules 2023, Vol. 28, Page 5979, 28(16), 5979. https://doi.org/10.3390/MOLECULES28165979

8. Fahmy MA, Z. S. H. M. W. A. (2019). Alteration in oxidative stress activities and trace elements levels on experimental Blastocystosis. Journal of Gastroenterology and Hepatology Research, 8(6), 3041–3048.