Ridwanto Ridwanto
Department of pharmacy, University Muslim Nusantara Al Washliyah, Medan, Indonesia, 20147

Zulmai Rani
Department of pharmacy, University Muslim Nusantara Al Washliyah, Medan, Indonesia, 20147

DOI: https://doi.org/10.19184/ams.v9i3.42614

Abstract

Yellow wood (Arcangelisia flava Merr and (L.)) is a medicinal plant that has receivedlittle attention. Yellow wood has antibacterial, antioxidant, anti-hyperlipidemic, and anti-cancer properties. Furthermore, yellow wood is known to contain a range of flavonoid compounds, such as hydroxylarcangelicin, which has broad antibacterial activity. The purpose of this research was to investigate the total flavonoid content of yellow wood extract as well as its antibacterial activity against Staphylococcus aureus bacteria. This step of research included the production of methanol extract, characterization of simplisia, phytochemical screening, and determination of the total flavonoid levels of the yellow wood methanol extract using a visual spectroscopic technique. Yellow wood methanol extract was made using the maceration method using methanol concentrations of 100%, 90%, and 80%, and the extract obtained was concentrated using a rotary evaporator. The total flavonoid content was then determined using the visible spectrophotometric method, and the antibacterial activity test was carried out against Staphylococcus aureus as a positive control. using chloramphenicol and a negative control using dimethyl sulfoxide (DMSO). The results of this study can be concluded to show that the total flavonoid levels in the methanol extract of yellow wood at a concentration of 80% were 228,314 ± 5,4171 mg QE/g. At a 90% concentration of 245.993 ± 6.7626 mg QE/g and a 100% concentration of 265.953 ± 3.0853 mg QE/g. The results of antibacterial activity at the concentration of 100% yellow wood methanol extract against Staphylococcus aureus bacteria have an average blandness of 12.65 mm.

Keyword: Yellow wood; Flavonoids; Spektrofotometri Visible; Staphylococcus aureus.

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