Husnarika Febriani
Biology Study Program, Faculty of Science And Technology, State Islamic University of North Sumatra, Deli Serdang, Indonesia

Syukriah
Biology Study Program, Faculty of Science And Technology, State Islamic University of North Sumatra, Deli Serdang, Indonesia

DOI: https://doi.org/10.19184/ams.v10i1.39415

Abstract

Tartrazine, as a synthetic food coloring consumed for a long time, has a toxic effect by free radicals in large quantities, creating an imbalance in the body and triggering disturbances, namely liver function. Free radicals can be minimized with antioxidants to reduce free radicals in the body. Antioxidants can be found in fruits, including Andaliman fruit. This study aimed to determine the effect of ethanol extract from Andaliman fruit on SGOT-SGPT levels and histology of tartrazine-induced white rats. The research design used 25 white rats with 5 treatments and 5 replications for 30 days. The negative control group was induced by 1% CMC, positive control induced by tartrazine 15 mg/kg BW, treatment groups 1, 2, 3 induced by tartrazine 15 mg/kg BW and ethanol extract andaliman fruit 150 mg/kg BW, 300 mg/kg BW, and 450 mg/kg BW. The stages in this study were screened for phytochemicals, total flavonoids and antioxidant activity, measurement of SGOT-SGPT levels, and observation of liver histology. Data were analyzed using one-way Anova and Duncan's further test. Results showed that phytochemical screening of Andaliman fruit extract contained flavonoids, terpenoids, steroids and tannins. The total flavonoid content in the extract was 7.8163 mgQE/g extract. The extract's antioxidant activity level has an IC50 value of 102.35 ppm. This study also showed that the extract affected decreasing levels of SGOT-SGPT and could minimize liver histological damage after being induced by tartrazine.

Keyword: Andaliman, tartrazine, SGOT-SGPT, liver histology.

REFERENCE

1. Amin, K.A., Hameid, A., and Elsttar, A. 2010. Effect Of Food Azo Dyes Tartrazine and Carmoisine On Biochemical Parameters Related To Renal, Hepatic Function And Oxidative Stress Biomarkers In Young Male Rats. Food And Chemical Toxicology.

2. Barhoma, R. A., Elshamy, A. M., and Amer, Alaa I. 2019. Study of the Protective Effect of Nigella Sativa Oil on Tartrazine-Induced Hematological Disorders In Rats. Med. J. Vol. 87 (7) : 4661-4670.

3. Batubara, M. S., Siregar, Y., Rusmarilin, H., Soviani, S., dan Febriani, Husnarika. 2017. Hubungan Kadar Tartrazin Dan Seng (Zn) Dalam Darah Pada Anak Penderita Defisit Perhatian Dan Gangguan Hiperaktivitas (ADHD). BioLink. Vol. 4 (1) : 1-10.

4. BPOM. 2019. Peraturan Badan Pengawas Obat dan Makanan Tentang Bahan Tambahan Pangan. Badan Pengawas Obat dan Makanan Republik Indonesia.

5. F. Alaa Ali., S.A. Sherein. A., S. Osama. El-Tawil., M. Adel, B. 2016. Toxicological And Hispathological Studies On The Effect Of Tartrazine In Male Albino Rats. International Journal Of Biological, Biomolecular, Agricultural, Food And Biotechnological Engineering. Vol. 10 (8) : 469-474.

6. Handajani, Fitri. 2021. Metode Pemilihan dan Pembuatan Hewan Model Beberapa Penyakit Pada Penelitian Eksperimental. Sidoarjo : zifatama Jawara.