Radinta Maharani Putri
Department of Parasitology Faculty of Medicine University of Jember, Jember, Indonesia

Erfan Efendi
Department of Parasitology Faculty of Medicine University of Jember, Jember, Indonesia

Elly Nurus Sakinah
Department of Parasitology Faculty of Medicine University of Jember, Jember, Indonesia

DOI: https://doi.org/10.19184/ams.v9i1.35312

Abstract

Diazinon, the most commonly used pesticide in Indonesia, increases the production of free radicals that trigger the lipid peroxidation process with the final product of MDA, which can damage cells and tissues, especially the kidneys. Neem gum, with its high polysaccharide, is a good antioxidant agent to neutralize free radicals. The purpose of this study is to determine the effect of neem gum administration to prevent the increase in kidney MDA levels of Wistar rats induced by diazinon. Thirty Wistar rats were divided into 6 groups, K0, K1 (DZN 40 mg/kgBW), P1, P2, P3, and P4 were administered neem gum as a drinking water at a dose of 3.75 g/kgBW, 7.5 g/kgBW, 15 g/kgBW, and 30 g/kgBW and induced by DZN 40 mg/kgBW. The treatment was carried out for 8 days, in which diazinon induction and neem gum solution were administered simultaneously. One Way ANOVA test showed a significant difference (p<0.05). The LSD posthoc test showed a significant difference in the treatment groups P2, P3, and P4 compared to the K1 group (p<0.05). This shows that the neem gum solution at a dose of 7.5 g/kgBW, 15 g/kgBW, and 30 g/kgBW could prevent the increase of kidney MDA level in Wistar rats induced by diazinon.

Keyword: Diazinon, Kidney, Oxidative stress, Antioxidant, Neem gum.

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