Wulan Usfi Mafiroh
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada

Anggiresti Kinasih
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada

Aulia Noor Ramadhani
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada

Rachmi Putri
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada

Endang Semiarti
Laboratory of Biotechnology, Faculty of Biology, Universitas Gadjah Mada

DOI: https://doi.org/10.19184/ams.v8i1.26848

Abstract

Orchids have been used as traditional herbal medicines in Indonesia. Orchids are able to produce secondary metabolites as their protective agent due to the extreme environment. Currently, molecular and metabolite data of orchids are available on the KNApSAcK and Dr. Duke's Phytochemical and Ethnobotanical Databases. Orchids have great diversity and potential to be the object of research in a computational study like in silico. In silico studies are affordable and can be used as preliminary research before in vitro or in vivo tests. The purpose of this study was to analyze the antimicrobial and anticancer potential of orchid secondary metabolites with web-based analysis through the online Prediction of Activity Spectra for Substance (PASS). This in silico study was carried out using Canonical SMILE of Vanilla spp., Dendrobium spp., and Vanda spp., secondary metabolite from KNApSAck-3D and Phytochem. After that, the antimicrobial activity and anticancer potential of the orchid secondary metabolites were analyzed using PASS online. The results showed that dendroside E from Dendrobium spp., and parviflorin from Vanda spp., have most potential antimicrobials activity than another secondary metabolites compounds. Parviflorin belongs to the annonaceous acetogenins that inhibit electron transport of ATP synthesis. Metabolite compound of 4-hydroxybenzoic acid from Vanilla planifolia has potential as an anticancer, especially as an inhibitor of chlordecone reductase.

Keyword: Orchid, antimicrobial, anticancer, secondary metabolite, PASS online.

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